NSJ Bioreagents

PD-L1 Antibody / B7-H1 / CD274

Product Code:
 
NSJ-V5622
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PDL1/8195R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant PD-L1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human HDLM-2 cell lysate using recombinant PD-L1 antibody (clone rCD274/8195R). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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Western blot testing of human HDLM-2 cell lysate using recombinant PD-L1 antibody (clone rCD274/8195R). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

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CodeSizePrice
NSJ-V5622-20UG20 ug£264.00
Quantity:
NSJ-V5622-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Application Note:
Optimal dilution of the recombinant PD-L1 antibody should be determined by the researcher.
Description:
PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant fragment (within amino acids 190-290) of human CD274 protein was used as the immunogen for the recombinant PD-L1 antibody.
Limitation:
This recombinant PD-L1 antibody is available for research use only.
Localisation:
Cytoplasm, Cell membrane
Purity:
Protein A/G affinity
Uniprot #:
Q9NZQ7