ProSci

HRG Antibody

Product Code:
 
PSI-23-579
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of mouse pancreas, using HRG antibody (23-579) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded mouse lung using HRG antibody (23-579) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse stomach using HRG antibody (23-579) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of mouse pancreas, using HRG antibody (23-579) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse lung using HRG antibody (23-579) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using HRG antibody (23-579) at dilution of 1:100 (40x lens).

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PSI-23-579-100uL100 uL£565.00
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Further Information

Additional Names:
HRG, DKFZp779H1622, HPRG, HRGP, THPH11
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-260 of human HRG (NP_000403.1).
NCBI Gene ID #:
3273
NCBI Official Name:
histidine-rich glycoprotein
NCBI Official Symbol:
HRG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P04196
User NOte:
Optimal dilutions for each application to be determined by the researcher.