Small RNA Prestain Marker Plus

For accurate electrophoretic determination of molecular weights, the Small RNA Marker (Cat # R0007) or Small RNA Marker Easy (Cat # R0008) should be used.
A migration of the Small RNA Prestain Marker Plus is optimized to use 10-15% acrylamide gel electrophoresis (see table 1).
This product is not for agarose gel electrophoresis.

The Small RNA Prestain Marker Plus consists of six prestained single-strand (blue and red) nucleic acids (apparent molecular weights are 20, 30, 40, 50, 75 and 100 bases) and it is visible during electrophoresis. The Small RNA Prestain Marker Plus is suitable for monitoring denaturing polyacrylamide gel electrophoresis and blotting onto membranes. The apparent sizes of bands in Small RNA Prestain Marker Plus are in excellent agreement with sizes of non-stained RNAs, 20, 30, 40, 50, 75 and 100 bases in length (about 95% accuracy). The Small RNA Prestain Marker Plus is supplied in a ready-to-use mixture and doesn't require heating or addition of a denaturing agent before use.

After 24-hrs incubation of the Small RNA Prestain Marker Plus at 37°C, no visible degradation of the marker is observed in 10% polyacrylamide - 7.5 M urea gel electrophoresis.

Recommended loading volumes:
Comb (Load volume)
4-10 mm (5-10 uL)
>10 mm (>10 uL)

In 2 mM Tris-HCl, pH 8.0 (8 mM EDTA, 78% formamide).