Human IFN-γ ELISA Kit

Interferon-gamma (IFN-γ) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-γ, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-γ is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and it is vital for the control of microbial pathogens. IFN-γ is believed to be crucial for host defense against many infections. Genetically determined variability in IFN-γ and expression might be important for the development of tuberculosis. IFN-γ activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-γ has important immunoregulatory functions. IFN-γ plays an important role in the control of neointima proliferation. The standard product used in this kit is recombinant human IFN-γ, consisting of 144 amino acids with the molecular mass of 17 KDa. ScienCell's human IFN-γ ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IFN-γ specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IFN-γ captured in strips.

For quantitative detection of human IFN-γ in serum, body fluids, tissue lysates or cell culture supernatants.