ProSci

PDL1 Detection Set

Product Code:
 
PSI-SD8500
Product Group:
 
Antibody Detection Sets
Supplier:
 
ProSci
Host Type:
 
Llama
Antibody Isotype:
 
sdab
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
Shipping:
 
dry ice
Storage:
 
PD-L1 antibodies should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 

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CodeSizePrice
PSI-SD8500-1Set1Set£313.00
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  • Further Information
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Further Information

Background:
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
Background References:
  • Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Conjugate:
Unconjugated
Immunogen:
PD-L1 antibodies were raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1.
Physical State:
Liquid
Purification:
PD-L1 Antibodies are affinity chromatography purified via Nickel column.Antibodies are supplied as a His-tagged purified protein.??They also contains a myc-tag for detection.
Set Contents:
20 μg:
PD-L1 Antibody [F6A9], Cat. No. SD8639
PD-L1 Antibody [F2G2], Cat. No. SD8641

100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
User Note:
Optimal dilutions for each application to be determined by the researcher. Antibodies have a C-terminal HIS 6-Tag followed by a myc-Tag for detection and have been tested using ProSci's PM-7669 (cMyc-tag Antibody [5G5H7]).

Documents

References

  1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  3. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.