Histone H3 Mutation Antibody Panel (K4M K9M K27M K36M) + H3.3 Mutation (G34W G34R G34V)

32160000

Avoid freeze/thaw cycles.

Contains 25µl of each antibody in this Antibody Panel Set

Histone H3.3/ H3 Antibody Panel reacts to Histone H3 K27M, K36M, K4M, K9M, along with H3.3 G34W, G34R, and G34V mutants. No cross-reactivity with wild-type Histone H3 has been shown. Western Blot: 1:125-1:1000ELISA: 1:125-1:1000Immunohistochemistry (IHC): 1:100-1:500. Contains 25µl of each antibody in this Antibody Panel Set.

Vial

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 has three main variants, H3.1 and H3.2, which are deposited in chromatin only during DNA replication and H3.3, which is replication independent and is found primarily in the regions of active transcription and heterochromatin. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone modifications are one form of epigenetic information that relate closely to gene regulation. Aberrant histone methylation caused by alteration in chromatin-modifying enzymes has long been implicated in cancers. Recently, recurrent histone mutations have been identified in multiple cancers and have been shown to impede histone methylation. All identified histone mutations (including H3K4M, H3K9M, H3K27M, H3K36M, and H3G34V/R/W) result in amino acid substitution at/near a lysine residue that is a target of methylation.

Serum

Histone H3.3/ H3 Antibody Panel reacts to Histone H3 K27M, K36M, K4M, K9M, along with H3.3 G34W, G34R, and G34V mutants. No cross-reactivity with wild-type Histone H3 has been shown. Western Blot: 1:125-1:1000ELISA: 1:125-1:1000Immunohistochemistry (IHC): 1:100-1:500

Non-hazardous

General Purpose Cell Biology Kits

41116111

Stable for at least 1 year after receipt when stored at -20°C.