H9-derived Neural Stem Cells

Includes 1 vial of frozen H9-NSC (Cat. #1660, 1 mL, >1 x 10^6 cells/vial), 50 mL Neural Induction Medium-basal (PSCNIM, Cat. #5931), 1 mL Neural Induction Medium Supplement 50x (PSCNIS, Cat. #5982)

Human Neural Stem Cells (H9-NSC) from ScienCell Research Laboratories are differentiated from a human pluripotent stem cell line (H9), which is human blastocyst derived. The monolayer of H9 cells are efficiently converted to neural epithelium using HPSC Neural Induction Medium (PSCNIM, Cat. #5931), a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (HPSCs). Dual modulation of Wnt and SMAD signaling pathway differentiates HPSCs to homogenous neural stem cells (NSC) within 7 days. The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: >95% of cells express Nestin and >95% of cells are SOX2 positive. H9-NSC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Cells are negative for mycoplasma, bacteria, yeast and fungi. After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture. NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages. To differentiate the H9-NSC, medium containing specific growth factors should be used (not provided).