Nucleus Pulposus Cell Medium

Nucleus Pulposus Cell Medium (HNPC), when used with Nucleus Pulposus Cell Growth Supplement (NPCGS, Cat #4852) and 10 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal nucleus pulposus cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal nucleus pulposus cells in vitro.

NPCM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of Nucleus Pulposus Cell Growth Supplement (NPCGS, Cat. No. 4852) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).