Adipocyte Medium

Adipocyte Medium (AdM), when used with Adipocyte Growth Supplement (AdGS, Cat #7262) and 12.5 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal adipocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2.5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal adipocytes in vitro.

AdM consists of 500 ml of basal medium, 12.5 ml of fetal bovine serum (FBS, Cat. No. 0012), 5ml of Adipocyte Growth Supplement (AdGS, Cat. No. 7262) and 5 ml of Antibiotic solution (P/S, Cat. No. 0503).