ScienCell's GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit (GQH-NLC) is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis. NSCLC accounts for more than 80% of all lung cancers. There are three main types of NSCLC: adenocarcinoma, lung squamous cell carcinoma and lung large cell carcinoma. Brief examples of how included genes may be grouped according to their functions are shown below:
- Oncogenes and tumor suppressor genes: - KRAS, HRAS, EML4, ALK, RARB, FHIT, RASSF1, TP53, CDKN2A
- Cell cycle regulation and apoptosis: - CDK4, CDK6, CCND1, CCNE2, CASP3, CASP9, BAD, STK4, FOXO3
- MAPK pathway: - KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: - PI3Ks, AKT1, AKT2
Genes related to specific lung cancer types -
- Adenocarcinoma: - ACTR3B, CADM1, ERBB2, CALB2, CDH1, EGF, FIGF, KRT20, NKX2-1, OGG1
- Lung squamous cell carcinoma: - CDH10, CYP1A1, FAM83B, FGFR1, GRAMD4, KRT19, PEBP1, PRKCI, S100A2, SOX2
- Lung large cell carcinoma: - CASK, CDC42BPB, DDR2, EPHA3, MYO18B, NME1, ROS1, TTN, WNK3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis.
