ScienCell's GeneQuery™ Human Cardiac Muscle Contraction and Cardiomyopathy qPCR Array Kit (GQH-CMC) contains primers for 88 genes involved in cardiac muscle contractions. Cardiomyocytes contain myofibrils, which shorten and lengthen to produce heart beats, partially relying on action potentials for input. An irregular heart beat may occur as a result of various heart muscle diseases. Brief examples of how included genes may be grouped according to function are shown below:
- Mitochondrial respiration: - AKR1B1, ANGPT2, HFE, NAGPA, VEGFA
- Sarcomere function: - SOD2, SYNM, TRPV1, GDNF, DBH, ACE
- Channels and transporters: - ATP1A1, SLC8A1
- Genes misregulated in cardiomyopathies: - ACE, CALR3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This array kit is designed to facilitate gene expression profiling of 88 key genes involved in cardiac muscle contractions.
