ScienCell's GeneQuery™ Human Osteoporosis qPCR Array Kit (GQH-POR) facilitates gene expression profiling of 88 genes involved in the establishment and progression of osteoporosis. Osteoporosis is a bone disease in which bone loss, lack of bone growth, or both result in weak and brittle bones. Complications of osteoporosis include broken bones and fractures. While osteoporosis affects everyone, older women are at higher risk of developing the disease. Brief examples of how included genes may be grouped according to their function are shown below:
- Bone remodeling: - ALOX15, COL1A1, COL1A2, IBSP, SP7
- Calcium regulation: - ALB, CALCR, PTH, CYP19A1, CALB1
- Osteoblast differentiation: - ALPL, RUNX2, TNFSF11, TNFRSF11A
- Cytokines and growth factors: - FGF23, BMP2, IGF1, TGFB1, LRP5
- Susceptibility markers: - AR, CASR, ESR2, CTSK, PDLIM4, VDR
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This array kit is designed to facilitate gene expression profiling of 88 key genes involved in the establishment and progression of osteoporosis.
