ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
- Pancreatic stellate cell markers: - ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF
- Pancreatic stellate cell activation: - ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2
- Retinol metabolism: - RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s
- ECM synthesis and remodeling: - TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
- Cytokine production: - IL1A, IL1B, IL6, PDGFs, TGFBs, TNF
- p53 signaling pathway: - CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3
- Pancreatic fibrosis and cancer development: - ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
This kit is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders.
