UK and Ireland Distributor of MBL International - Reagents for General Research Use

Reagents for Research Use

MBLI develops, manufactures, and markets a broad portfolio of monoclonal and polyclonal antibodies, fluorescent and recombinant proteins and ELISA kits for research.

Products can be classified into a number of research areas. Listed within each section below is a highlight of the current most popular products

• Epitope Tags
• Cancer
• Kinase
• Tetramer
• Immunology
• Epigenetics
• Autophagy
• Apoptosis
• Fluorescent Proteins
• Metabolism
• Neuroscience

Epitope Tags

MBLI provide a variety of polyclonal and monoclonal antibodies for isolation of tagged proteins and analysis of protein protein interactions. Their line of fusion protein and epitope antibodies are available as unlabeled and conjugated to multiple fluorophores, agarose, magnetic beads and magnetic agarose for a variety of applications.

MBLI has developed and designed new monoclonal antibodies with high sensitivity that:

• Recognizes the tag at any location (N-Terminal, C-Terminal and Internal domain)
• Single antibody multiple applications (WB, IPP, ICC, FC)
• High sensitivity & specificity

Anti-Myc-tag mAb (Clone: My3) Product Code M192-3

Anti-His-tag mAb (Clone: OGHis) Product Code D291-3

Anti-DDDDK-tag mAb (Clone: FLA-1) Product Code M185-3

Epitope tags available from MBL

Tag Protein Purification

MBLI offers a wide range of anti-tag purification such as columns, gels and protein purification kits. MBLI purification kits are designed for the isolation of tagged proteins from cell lysates and cell culture supernatants. Using anti-tag antibodies conjugated to beads, the tagged proteins are quickly and efficiently purified using a spin column. Their kits are optimized to maintain protein activity and conformity using neutral pH conditions.

DDDDK-tagged Protein purification cartridges 1 x 1 mL, Product Code 3326K

V5-tagged protein purification kit, 20 test, Product Code 3315

His-tagged Protein purification kit, 20 test, Product Code 3310

c-Myc-tagged Protein purification kit, 20 test, Product Code 3305

HA-tagged Protein purification kit, 20 test, Product Code 3320

Cancer

P53

Request a P53 in cancer poster

P53 is an important factor for tumour suppression and is activated under cellular stress (e.g., DNA damage) to induce cell cycle arrest or apoptosis. p53 mutants found in tumours are no longer functional as tumour suppressors, resulting in cell malignancies. The immune response in tumour patients correlates with the presence of p53 mutations, which leads to the accumulation of ineffective p53 proteins in the tumour cells. Anti-p53 autoantibodies are produced in many types of tumours and can be detected during early tumour stages compared to conventional tumour marker detection. Autoantibody signatures in cancer, such as anti-p53 antibodies, are potential tumour biomarkers.

Anti-p53 ELISA Test Kit, 48 well, Product Code RG-7640E

IDH antibodies

Human isocitrate dehydrogenases (IDHs) are classified into three subtypes: IDH1, IDH2 and IDH3. IDH1 mutations have been found in patients with acute myeloid leukaemia, while IDH1/2 mutations have been found in multiple stages of brain tumours such glioma and glioblastoma. The mutant forms of IDH1 appear in 90% of patients with glioma while the mutant forms of IDH2 appear in 50% of patients, making IDH1/2 mutations a useful marker for prognosis of glioma. MBLI offers highly specific antibodies to mutant forms of IDH1 (R132H, R132S, R132G) and IDH2 (R172K, R172M, R172W) which can be used to detect mutated IDHs in specimens of low tumour occupancy.

Human Reg4 ELISA Kit

• Secreted protein, function is unknown
• Up-regulated in pancreatic cancer, colon cancer

FAM3D Human Assembly ELISA Kit 96 well, Product Code 5321

NGAL Human Assembly ELISA Kit 96 well, Product Code 5322

MICA Human Assembly ELISA Kit 96 well, Product Code 5330

MICB Human Assembly ELISA Kit 96 well, Product Code 5331

WFDC2 (HE4) Human Assembly ELISA Kit 96 well, Product Code 5335

LYPD6B Human Assembly ELISA Kit 96 well, Product Code 5336

REG4 Human Assembly ELISA Kit 96 well, Product Code 5337

Elafin (SKALP) Human Assembly ELISA Kit 96 well, Product Code 5338

ST2 Human ELISA Kit 96 well, Product Code 7638

SIRT1/Sir2 Deacetylase Fluorometric Assay Kit 100 assay, Product Code CY-1151

S100P ELISA Kit

• In recent publications, S100P was highly expressed in tissues of patients with pancreatic cancer.
• Detects S100P in serum, plasma, cell lysate and cell culture supernatants.

S100P ELISA Kit 96 assay, Product Code CY-8060

S100A10 ELISA Kit 96 assay, Product Code CY-8095

Kinase

AMP-Activated Protein Kinase (AMPK) regulates cellular energy metabolism by modulating the activity of key enzymes such as acetyl-coenzyme A carboxylase (ACC) and mTOR (mammalian target of rapamycin) in metabolic pathways through their phosphorylation and of transcription factors and cofactors. AMPK stimulates energy-producing pathways (e.g., glucose transport, fatty acid oxidation), and turns off energy-consuming pathways (e.g., protein synthesis, lipogenesis, gluconeogenesis). Adiponectin and leptin as well as hypoglycemic drugs are known as activators of AMPK in adipose tissues. The activation of AMPK occurs upon fasting and exercise which involve changes in the AMP/ATP ratio and the primary upstream liver kinase B1 (LKB1). AMPK is the target of metformin and hormones which improve insulin sensitivity and may serve as the target of pharmaceuticals involved in insulin resistance (type 2 diabetes).

Cyclic GMP dependent protein kinase (cGK) Assay Kit 96 well, Product Code CY-1161

AMP Kinase Assay Kit 96 assay, Product Code CY-1182

CaM-Kinase II Assay Kit 96 well, Product Code CY-1173

Rho-Kinase Assay Kit 96 well, Product Code CY-1160

Serine/Threonine Kinase Assay Kits

AKT/PKB, AMPK, Aurora, Aurora family, CaM Kinase II, Cdc-Cyclin B, CK2, Checkpoint kinase, cGK, DYRK2, IKKαandβ, MAPKAP-Kinase 2, Human Mps1/TKK, p38, PDK1, Pim-1, PKC Super Family, Polo-like Kinase 1, Polo-like Kinase 2, Polo-like Kinase 3, Rho-Kinase

Tyrosine Kinase Assay Kits

c-Src, Eph A2, FGFR2, Met, Lck/p56, Pyk2, TrkA, Wee1

Phosphatase Assay Kits

Cdc25A, Cdc25B, Cdc25C, Cdc25 Combo, Cdi/KAP, LMW-PTP/ACP1, PTP1B, TC-PTP

Tetramer

MBLI has a wide portfolio of immune monitoring research products including tetramer technology for vaccine development, rapid T-cell detection and quantification in the pharmaceutical industry or bioresearch industry.

Major Histocompatibility Complex (MHC) tetramer technology utilizes the MHC/peptide complex to detect antigen specific T cells at the single-cell level, thus surpassing detection limits of traditional cell-based methods. MHC tetramer technology is the most accurate method for detection of rare events. The information MHC tetramers provide is pivotal the direct development of vaccines and therapies, as well as monitoring patient response during clinical trials. To greatly reduce non-specific binding, Class I iTAgTM MHC Tetramers specific to human alleles have a proprietary mutation in the α3 domain.
Read more here...

Immunology

Graft-versus-host disease (GVHD) is a common complication associated with HSCT. GVHD occurs when transplanted immune cells recognize the recipient as "foreign" creating an immune response where the transplanted cells attack the host cells. In addition to providing MHC Tetramers.

 which can be used to monitor assess CMV risk in transplant patients, MBLI offers several GVHD biomarkers including: CRP, Elafin, IL-18, Lactoferrin, REG3alpha and ST2.

High-Sensitivity CRP ELISA Kit 96 assay, Product Code CY-8071

Human Elafin (SKALP) assembly ELISA Kit 96 well, Product Code 5338

Human IL-18 ELISA Kit 96 well, Product Code 7620

Human Lactoferrin ELISA Kit 96 assay, Product Code CY-8089

Human PAP1 (REG3α) assembly ELISA Kit 96 well, Product Code 5323

ST2 ELISA Kit 96 well, Product Code 7638

Interleukin-18 (IL-18) is a cytokine produced by dendritic cells, monocytes, macrophages, neutrophils, and epithelial cells. Th1-type immunoreactions are facilitated primarily by IL-18. Furthermore, action of IL-18 on basophils and mast cells cause complex physiological responses, including allergic reactions. High levels of IL-18 are detected in the blood of patients with allergic and autoimmune diseases. IL-18 is also found in the urine of patients with acute renal disorder and mediates the progression of type 2 diabetes.

Human IL-18 ELISA Kit, 96 well, Product Code 7620

Mouse IL-18 ELISA Kit, 96 well, Product Code 7625

Eosinophil Cationic Protein (ECP) and Eosinophil Derived Neurotoxin (EDN) and Major Basic Protein (MBP) are known to be major protein-mediators derived from activated eosinophils. Activated eosinophils play an important role in inflammatory processes especially in allergic diseases. The levels of eosinophil activation can serve as complimentary data for monitoring asthma inflammation. As ECP/EDN proteins are secreted from activated eosinophils, they become markers of eosinophil activation and degranulation which can cause inflammatory disease of the airways.

ECP

• No cross reactivity with EDN
• For use with human serum samples

ECP ELISA Kit, 96 well, Product Code RG-7618EC-D (CE marked)

EDN

• No cross reactivity with ECP
• For use with human serum samples

EDN ELISA Kit, 96 well, Product Code 7630

Epigenetics

MethylHunter™ DNA Enrichment Kit. MBD1 binds to methylated CpG DNA through its N-terminus MBD. MethylHunter™ DNA Enrichment Kit enriches DNA fragments containing methylated CpG DNA by using recombinant His-tagged MBD1.

MBD1-based methylation DNA Enrichment Kit

• High enrichment efficiency
• Consistent enrichment throughout genome loci
• Quick and easy protocol

MethyIHunter MBD1-based Methylated DNA Enrichment Kit 25 test, Product Code 5270

MethyIHunter MBD1-based Methylated DNA Enrichment with beads 25 test, Product Code 5275

Post-transcriptional regulation of gene expression is important in most cellular processes.RNP complexes are the fundamental units of post-transcriptional regulation of mRNA. RNA molecules, including mRNA and non-coding species such as microRNA (miRNA), exist in RNP complexes with specific RNA-binding proteins (RBPs).

Discover RNA Binding Proteins

• Immunoaffinity method to explore RNA proteins and RNA-RNA interactions
• Reliable method to describe the endogenous assembly of ribonucleoproteins
• Identify regulatory components in biosynthesis of small RNA and non-coding RNA

RiboTrap Kit 10 assay, Product Code RN1011 and RN1012

RIP-Assay Kit

• Analyze intact RBP-mRNA complexes
• Identify miRNAs bound to mRNA targets
• Analyze the maturation mechanism of miRNA
• Identify RISC components and analyse their function
• Identify functionally related RNA libraries

RIP-Assay Kit 10 assay, Product Code RN1001

RIP-Assay Kit for microRNA 10 assay, Product Code RN1005

Anti-ELAVL1 (HuR) (Human) pAb 200 uL, Product Code RN004P

Anti-EIF4G1 (Human/Mouse) pAb 200 uL, Product Code RN002P

Anti-EIF4E (Human) pAb 200 uL, Product Code RN001P

Anti-EIF2C2 (AGO2) (Human) mAb 200 uL, Product Code RN003M

Autophagy

Request an autophagy poster

Autophagy is a cellular self-digestion process for the purpose of providing nutrients to enable cells to survive starvation. Autophagy plays an important role in maintaining the cell homeostasis by removing cellular components that have degraded. Recent research has also shown an association of mammalian autophagy with pathological conditions including neurodegenerative diseases, infection, cardiac disease, cancer and aging.

LC3 plays an integral role as an autophagosome maker, because it resides in the entire process of autophagosome formation. Upon autophagy induction, LC3-I, a small and soluble cytoplasmic protein, is conjugated with a phospholipid which anchors it to the autophagosomal membrane as LC3-II. Autophagy can be induced by nutrient deprivation. Our polyclonal and monoclonal antibodies can be used to quantify LC3-I and LC3-II levels within the cell.

LC3 Polyclonal, WB, IPP, ICC,IHC,FC, Product Code PM036

LC3 Monoclonal (Clone: 4E12), WB,IPP, ICC, FC, Product Code M152-3

LC3 Monoclonal (Clone: 8E10), WB, Product Code M186-3

Apoptosis

Request an apoptosis poster

Apoptosis Detection Kits - Caspase Activity Measurement

Caspase-3 Colorimetric Assay Kit 100 assay, Product Code 4800

Caspase-3 Fluorometric Assay Kit 100 assay, Product Code 4815

Caspase-8 Colorimetric Assay Kit 100 assay, Product Code 4805

Caspase-8 Fluorometric Assay Kit 100 assay, Product Code 4820

Caspase-9 Colorimetric Assay Kit 100 assay, Product Code 4810

Caspase-9 Fluorometric Assay Kit 100 assay, Product Code 4825

Cathepsin S Fluorometric Assay Kit 100 assay, Product Code CY-1250

Apoptosis Detection Kits - TUNEL Based Detection

Apoptosis Kit Direct 66(FCM)/40(IHC), Product Code 8445

Apoptosis Kit-II 66(FCM)/40(IHC), Product Code 8441

Apoptosis Detection Kits - Annexin V Detection

Apoptosis Kit 100 assay, Product Code 4700

Annexin V-Azami-Green Apoptosis Detection Kit 100 assay, Product Code 4690

Fluorescent Proteins

FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enable the visualization of cell cycle progression in living cells. FUCCI takes advantage of the fact that the replication licensing factors Cdt1 and Geminin are only present during specific phases of the cell cycle. A fusion protein of a fragment of Cdt1 (amino acids 30-120) with the fluorescent protein monomeric Kusabira-Orange 2 (mKO2) serves as an indicator of G1 phase, while a fusion protein of a fragment of Geminin (amino acids 1-110 or 1-60) with the fluorescent protein monomeric Azami-Green 1 (mAG1) visualizes S, G2 and M phase. The cell cycle indicator takes advantage of the highly selective, rapid degradation of the replication licensing factors, mediated by the ubiquitin-proteasome system.

• Used for imaging the spatiotemporal patterns of cell cycle dynamics
• FACS - Sort your cells by cell cycle phase
• In Vivo analysis

FUCCI Cloning vector set (G1 Orange + S/G2/M Green) Product Code AM-VS0601

FUCCI Cloning vector set (G1 Orange + S/G2/M Green(N+C)) Product Code AM-VS0605

FUCCI Expression vector set (G1 Orange + S/G2/M Green-Hyg) Product Code AM-VS0607

FUCCI Expression vector set (G1 Orange + S/G2/M Green (N+C)-Hyg) Product Code AM-VS0608

Metabolism

Request a cholesterol-cardiovascular pathway poster

PCSK9 is an extracellular protein expressed primarily in the kidneys, liver and intestines representing the 9th member of the secretory subtilase family. PCSK9 plays a major role in cholesterol metabolism through posttranslational down-regulation of LDLR, the receptor responsible for clearing LDL-C from plasma. PCSK9 binds directly to first epidermal growth factor-like repeat A (EGF-A) domain of LDLR and is required for LDLR degradation. PCSK9 would seem to be an attractive drug target for lowering LDL-C.

Human PCSK9 ELISA Kit 96 well, Product Code CY-8079

PCSK9-LDLR 96 well, Product Code CY-8150

Human Adiponectin ELISA Kit 96 well, Product Code CY-8050

Human Chitotriosidase ELISA Kit 96 well, Product Code CY-8074

Chitotriosidase Fluorometric Assay Kit 100 assay, Product Code CY-1249

Neuroscience

Request an Alzheimer's pathway poster

MBLI offers over 7,000 antibodies, proteins and kits, many of which are related to neuroscience.

Popular targets such as ApoE4 and the S100 proteins are commonly used for Alzheimer's, Cystic fibrosis and other neurological disease research.

Anti-Reelin/CR-50 mAb, Product Code D223-3

Anti-Amyloid beta mAb, Product Code M046-3

S100 proteins are a family of calcium binding proteins, where each member has specific structure and function. S100 proteins have been observed to play a role in various diseases, making these proteins an important marker for disease diagnosis including possible therapeutic targets.

ApoE4/Pan-ApoE ELISA Kit 96 well, Product Code 7635

DJ-1/PARK7 ELISA Kit 96 well, Product Code CY-9050

S100A12/EN-RAGE ELISA Kit 96 well, Product Code CY-8058

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